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Dr. Subodh Kumar Sinha

Dr. Subodh Kumar Sinha
Principal Scientist
subodh[dot]sinha[at]icar[dot]gov[dot]in
Phone :
9990112646

Discipline: Molecular Biology and Biotechnology

Education:

Ph.D.: Biochemistry, Indian Institute of Agricultural Research, Pusa, New Delhi

Research Interest

  • Nitrogen use efficiency (NUE) is a complex trait which needs to be improved for agriculturally important crops in order to minimize N-fertilizer cost and environmental degradation caused due to unused applied-N in the soil. In order to improve nitrogen use efficiency of wheat, we follow nitrate-uptake mediated improvement of NUE in wheat. Currently my laboratory undertakes research on identification and functional characterization of key nitrate uptake and translocation related genes in bread wheat. We are also interested to identify partner proteins of two-component high affinity nitrate transport system in bread wheat.

 

 

 

Awards & Honors

  • Visiting Scientist-Department of Plant Sciences, University of Cambridge, Cambridge, UK. 
  • Visiting faculty at Advanced Centre for Agricultural Research & Education, Yezin Agricultural University, Myanmar
  • Best Oral Presentation Award (Molecular Breeding and Genomics Session) in National Conference on Integrative Plant Biochemistry and Biotechnology, organized by ICAR-IIRR, Hyderbad and SPBB New Delhi, 2019. 
  • Best Oral Presentation Award: International Conference on Life Sciences, Informatics, Food, and Environment. Organized by JIIT, Noida, 2014.
  • Best Teacher award- Rajendra Agricultural University, Bihar: 2010
  • Summer Research fellowship (2008) by Indian Academy of Sciences, Indian National Science Academy & the National Academy of Sciences.
  • Graduate Aptitude Test in Engineering –2004 (GATE 2004). Percentile Score-93.07 in Life sciences
  • Junior Research Fellowship (UGC-JRF) award for doctoral study in life sciences
  • Junior Research Fellowship (ICAR-JRF) award for M.Sc program in IARI by Indian Council of Agricultural Research (2000).
  • Kumari P, Kumar A, Kumar S, Singh PK, Mandal PK, Das TK, Sinha SK (2023) The physio-molecular responses of bread wheat (Triticum aestivum L.) induced by coexisting Phalaris minor Retz. under nitrate-limiting conditions: perspectives of kin and non-kin recognition. J Soil Sci Plant Nutr. https://doi.org/10.1007/s42729-023-01283-7
  • Paul D, Satuluri S,Sinha SK (2023) Nitrogen assimilation and fractationation dynamics in wheat genotypes grown in different mediums. J. Plant Growth Regul. https://doi.org/10.1007/s00344-023-11020-0
  • Dutta H, Mishra GP, Shivaprasad KM, Sinha SK, Aski MS, Vijay D, Prasad M, Das S, Pawar PA-M, Mishra DC, Singh AK, Kumar A, Tripathi K, Kumar RR, Gupta S, Kumar S and Dikshit HK (2023), Morpho-biochemical characterization of a RIL population for seed parameters and identification of candidate genes regulating seed size trait in lentil (Lens culinaris Medik.). Front. Plant Sci. 14:1091432. doi: 10.3389/fpls.2023.1091432
  • Kumar A, Shamnas Muhhamed v, Jayaswal PK, Mahato AK, Arya A, Mandal PK, Singh NK, Sinha SK (2023) Growth stage and nitrate limiting response of NRT2 and NAR2 gene families of bread wheat, and complementation and retrieval of nitrate uptake of antnrt2.1 mutant by a wheat NRT2 gene. Environ Exp Bot. DOI: 10.1016/j.envexpbot.2022.105205
  • Dutta H, Mishra GP, Aski MS, Bosamia TC, Mishra DC, Bhati J, Sinha SK, Vijay D, C. T. MP, Das S, Pawar PA-M, Kumar A, Tripathi K, Kumar RR, Yadava DK, Kumar S and Dikshit HK (2022), Comparative transcriptome analysis, unfolding the pathways regulating the seed-size trait in cultivated lentil (Lens culinaris Medik.). Front. Genet.. 13:942079. doi: 10.3389/fgene.2022.942079
  • Amresh Kumar, Sarvendra Kumar, Karnam Venkatesh, Nagendra Kumar Singh, Pranab Kumar Mandal, Subodh Kumar Sinha (2022) Physio-molecular traits of contrasting bread wheat genotypes associated with 15N influx exhibiting homeolog expression bias in nitrate transporter genes under different external nitrate concentrations. Planta. 255:104. https://doi.org/10.1007/s00425-022-03890-7
  • Priyanka Kumari, Loitongbam Lorinda Devi, Amresh Kumar, Ashutosh Pandey, Subodh Kumar Sinha , Amar Pal Singh ( 2022 ) Differential response of rice genotypes to nitrogen availability is associated with the altered nitrogen metabolism and ionomic balance. Environmental and Experimental Botany, 198:104847. https://doi.org/10.1016/j.envexpbot.2022.104847
  • AlkaBharati, GayatriTehlan, ChetanKumarNagar, SubodhKumarSinha, KarnamVenkatesh, PranabKumarMandal (2022) Nitrogen stress‑induced TaDof1 expression indiverse wheat genotypes andits relation withnitrogen use efciency. Cereal Research Communications. https://doi.org/10.1007/s42976-021-00227-0
  • Sarvendra Kumar, Anil Sharma, Rosin KG, Vinod K Sharma, Subodh Kumar Sinha, Dinesh Kumar, Neeta Dwevedi, Vishwanath (2021) Medium-term Impact of Organic and Microbial Fertilization on Production Efficiency and Fertility of Soil in an Apple Production System under North West Himalayan Region. Communications in Soil Science and Plant Analysis, DOI: 10.1080/00103624.2021.2008416

  • T. Danakumar, Jyoti Kumar, Amit Kumar Singh, Subodh Kumar Sinha, Anjan Kumar Pradhan, Shivani Sharma, Shailendra Kumar Jha, Ruchi Bansal, Sundeep Kumar, Girish Kumar Jha, Mahesh C. Yadav, P.V. Vara Prasad (2021) Genetic Dissection of Seedling Root System Architectural Traits in a Diverse Panel of Hexaploid Wheat through Multi-Locus Genome-Wide Association Mapping for Improving Drought Tolerance. Int. J. Mol. Sci. 7188;https://doi.org/10.3390/ijms22137188

  • Amitha Mithra Sevanthi, Subodh Kumar Sinha, Suresh Kumar V, Manju Rani, Manish Ranjan Saini, Sapna Kumari, Megha Kaushik, Chandra Prakash, Venkatesh K, G. P. Singh, Trilochan Mohapatra, Pranab Kumar Mandal (2021) Integration of Dual Stress Transcriptomes and Major QTLs from a Pair of Genotypes Contrasting for Drought and Chronic Nitrogen Starvation Identifies Key Stress Responsive Genes in Rice. Rice, 14-49.

  • Moring A,……… Sinha, SK,…….and Sutton MA (2021) Nitrogen Challenges and Opportunities for Agricultural and Environmental Science in India. Front. Sustain. Food Syst. 5:505347. doi: 10.3389/fsufs.2021.505347

  • Gayatri, Karikalan Jayaraman, Subodh Kumar Sinha, Pranita Roy, Pranab Kumar Mandal (2021) Comparative analysis of GS2 and Fd-GOGAT genes in cultivated wheat and their progenitors under N-stress. Plant Mol. Biol. Rep. https://doi.org/10.1007/s11105-020-01267-2

  • Uttarayan Dasgupta; Gyan Prakash Mishra; Harsh K. Dikshit; Dwijesh C. Mishra; Tejas Bosamia; Anirban Roy; Jyotika Bhati; Priti .; Muraleedhar Aski; Ranjeet R. Kumar; Amit K. Singh; Atul Kumar; Subodh Kumar Sinha. Sinha; Shiksha Chaurasia; Shelly Praveen; Ramakrishnan M. Nair (2021) Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean (Vigna radiata L.). PLoS ONE. Accepted.

  • Vishwanath., Sarvendra Kumar, Tapan Jyoti Purakayastha, Siba Prasad Datta, Rosin K.G., Prabhakar Mahapatra, Subodh Kumar Sinha and Surya Prakash Yadav (2020) Impact of forty-seven years of long-term fertilization and liming on soil health, yield of soybean and wheat in an acidic Alfisol, Archives of Agronomy and Soil Science, DOI: 10.1080/03650340.2020.1843023

  • Megha Kaushik, Shubham Rai, Sureshkumar Venkadesan, Subodh Kumar Sinha, Sumedha Mohan, Pranab Kumar Mandal (2020) Transcriptome Analysis Reveals Important Candidate Genes Related to Nutrient Reservoir, Carbohydrate Metabolism, and Defence Proteins during Grain Development of Hexaploid Bread Wheat and Its Diploid Progenitors. Genes (MDPI), 11:509. Doi. doi:10.3390/genes11050509

  • Subodh Kumar Sinha, Amresh Kumar, Akanksha Tyagi, Karnam Venkatesh, Debajyoti Paul, Nagendra Kumar Singh, Pranab Kumar Mandal (2020) Root architecture traits variation and nitrate-influx responses in diverse wheat genotypes under different external nitrogen concentrations. Plant Physiology Biochemistry. 148:246-259. https://doi.org/10.1016/j.plaphy.2020.01.018

  • Subodh Kumar Sinha,Akanksha Tyagi, Pranab Kumar Mandal(2019)External Nitrogen and Carbon Source Mediated Response on Modulation of Root System Architecture and Nitrate Uptake in Wheat Seedlings.J. Plant Growth Regul.38:283-297DOI 10.1007/s00344-018-9840-9

  • Shubham Rai, Megha Kaushik, Subodh Kumar Sinha, Rajesh Kumar Gupta, Anju Mahendru, Pranab Kumar Mandal (2018) Transcriptome Data of Cultivated Tetraploid and Hexaploid wheat variety during grain development. Data in Brief. 22:551-556.

  • Gayatri, Manju Rani, Ajay Kumar Mahato, Subodh Kumar Sinha, Monika Dalal, Nagendra Kumar Singh, Pranab Kumar Mandal (2018) Homeologue Specific Gene Expression Analysis of Two Vital Carbon Metabolizing Enzymes—Citrate Synthase and NADP-Isocitrate Dehydrogenase—from Wheat (Triticum aestivum L.) Under Nitrogen Stress. Applied Biochem. Biotechnol. doi.org/10.1007/s12010-018-2912-2

  • Sharda Bharti, Subodh Kumar Sinha (2018) Biochemical Characterization of Building Block of Condensed Tannin in Faba Bean (Vicia faba L.). Vegeta's 31 (4):25-27. Doi: 10.5958/2229-4473.2018.00088.5

  • Subodh Kumar Sinha, Manju Rani, Amresh Kumar, Sarvendra Kumar, K. Venkatesh, Pranab Kumar Mandal (2018) Natural variation in root system architecture in diverse wheat genotypes grown under different nitrate conditions and root growth media. Theor. Exp. Plant Physiol.DOI 10.1007/s40626-018-0117-2

  • Chetan Kumar Nagar, Gayatri, Alka Bharti, Subodh Kumar Sinha, K. Venkatesh, Pranab Kumar Mandal (2018) Nitrogen Stress Leads to Induce Change in Expression of Genes for Nitrate Transporter in Wheat Genotypes. Int. J. Curr. Microbiol. App. Sci. 7(6): 2991-3001.

  • Subodh Kumar Sinha, Amitha Mithra Sevanthi V, Saurabh Chaudhary, Punit Tyagi, Sureshkumar Venkadesan, Manju Rani, Pranab Kumar Mandal (2018) Transcriptome analysis of two rice varieties contrasting for nitrogen use efficiency under chronic N starvation reveals differences in chloroplast and starch metabolism-related genes. Genes9:206. doi:10.3390/genes9040206.

  • Subodh Kumar Sinha, Amresh Kumar (2018) Condensed tannin: a major anti-nutritional constituent of faba bean (Vicia faba L). Horticult Int. J. 2(2):32-33.

  • Subodh Kumar Sinha, Manju Rani, Niketa Bansal, Gayatri, K Venkatesh, and PK Mandal (2015) Nitrate Starvation Induced Changes in Root System Architecture, Carbon-Nitrogen Metabolism and miRNAs Expression in Nitrogen Responsive Wheat Genotypes. Appl Biochem Biotechnol.177: 1299-1312.

  • Amresh Kumar, Nidhi, N. Prasad, and Subodh K. Sinha (2015) Nutritional and antinutritional attributes of Faba bean (Vicia faba L.) growing in Bihar, India. Physiol. Mol. Biol. Plants. 21:159-162.

  • Pradhan A, Shahi VK, Sinha SK (2014) Evaluation of Genetic Diversity in Faba bean (Vicia faba L.) Genotypes using Seed Protein and Isozymes Electrophoresis. Natl. Acad. Sci. Lett. 4:303-309.

  • Sinha SK, Kumar M, Kumar V, Bharti S, Shahi VK (2013) Antioxidant activities of different tissue extract of Faba bean (Vicia faba L.) containing phenolic compounds. Legume Res. 36(6):496-504.

  • Smita S, Sinha SK, Sharma, Shahi VK (2011) Phenotypic and physiological characterization of rhizobia strains isolated from different areas of Bihar. Bioscience Discovery 2: 281-287.

  • Sinha SK (2010) RNAi induced gene silencing in crop improvement. Physiol. Mol. Biol Plants. 16:321-332.

  • Smita S, Sinha SK, Dayaram, Shahi VK (2010) Molecular diversity of rhizobia strains isolated from different areas of Bihar. RAU J. Res.20 (1&2) 1-5.

  • Gupta NC, Sinha SK, Jolly M, Dubey N, Sachdev A (2009) Antisense RNA-Mediated Inhibition of GmFAD2-1 Encoding Omega-6-Desaturase.Indian J. Plant Physiol. 14: 336-343.

  • Sinha SK, Dubey N, Sachdev A (2008) Gene silencing constructs for alteration of seed fatty acid composition by RNAi induced transgene silencing. New Botanist 35: 23-32.

  • Kishore K, Sinha SK, Kumar R, Gupta NC, Dubay N, Sachdev A (2007) Isolation and characterization of microsomal ω-6 desaturase gene (fad2-1) from soybean. Indian J. Expt. Biol. 45: 390-397.

  • Kumar R, Kishore K, Sinha SK, Gupta NC, Dubay N, Sachdev A (2007) Isolation and characterization of fad2-1 cDNA sequence from Glycine max L. Indian J. Plant Physiol. 12: 6-12.

  • Sinha SK, Dubey N, Sachdev A (2006) Intron-spliced gene silencing construct for alteration of seed fatty acid composition by RNAi induced transgene silencing. New Botanist 33: 181-191.

  • Sinha SK, Dasgupta A, Baranwal A, Praveen P (2004) Molecular Variability in the replicase gene of viruses causing tomato leaf curl disease in India. J Plant Biochem. Biotechnol. 13: 43-46.

  • Dasgupta A, Sinha SK, Praveen P (2004) Structure of replication initiator protein unites diverse viruses causing tomato leaf curl disease (ToLCD). Plant Sci. 166: 1063-67.

  • Praveen P, Kulkarni MS, Sinha SK, Ahlawat YS (2004) Diagnosis of Tomato leaf curl virus (ToLCV) by Polymerase Chain Reaction (PCR). Indian Phytopathology.57: 117-120.

  • Praveen P Dasgupta A, Sinha SK, Varma A (2003) Structure of a replication initiator protein of Tomato leaf curl virus. Indian Phytopathology. 56: 504.

Book Chapter:

  • Subodh Kumar Sinha and Basavaprabhu Patil. Applications of RNA-interference and Virus Induced Gene Silencing (VIGS) for Nutritional Genomics in Crop Plants. In Phytonutritional Improvement of Crops. Ed. Noureddine Benkeblia. Willy-Blackwell. 2017 pp. ISBN: 978-1-119-07994-1
  • Subodh Kumar Sinha, S. V. Amitha Mithra and Pranab Kumar Mandal. Nitrogen Use Efficiency in Cereals-Biotechnological Interventions. In Progress of Biotechnology in India. Eds S. C. Santra and Anusaya Mallick. ENVIS Centre on Biotechnology, University of Kalyani, Kalyani, West Bengal. 2017 pp. 63-86. ISBN: 978-93-5267-783-2 (HB) ISBN: 978-93-5267-784-9 (PB)
  • Subodh K. Sinha, R. Srinivasan and P.K. Mandal. MicroRNA-Based Approach to Improve Nitrogen Use Efficiency in Crop Plants. In Nutrient Use Efficiency: from Basics to Advances Eds A. Rakshit, A. Sen and H.B. Singh. Springer New Delhi Heidelberg New York Dordrecht London 2015 pp. 221-235.
  • Subodh K. Sinha, Om Prakash Gupta, K. Venkatesh and P. K. Mandal. Micro RNA Mediated Gene Regulation of Nutrient Homeostasis in Plants. In Recent Advances in Biotechnology: Plant Biotechnology Vol. 2 Ed. P. Ananda Kumar. Studdium Press LLC USA 2014 pp.71-89.
  • Subodh Kumar Sinha. Faba Bean: Nutritional Values and Prospects for Trait Improvements. In Advances in Plant Physiology Vol. 15 Ed. A. Hemantaranjan. Scientific Publisher (India), Jodhpur, 2014 pp.:402-416.
  • Subodh K. Sinha and N. Prasad. Antinutritional Constituents in Grain Legumes. In Advances in Plant Physiology Ed. A. Hemantaranjan. Scientific Publisher (India), Jodhpur, 2009 pp.:421-436. (ISSN: 0972-9917; ISBN: 978-81-7233-631-8)