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Dr. Rohini Sreevathsa

Dr. Rohini Sreevathsa
Principal Scientist
Rohini[dot]sreevathsa[at]icar[dot]gov[dot]in
Phone :
9990964333

Discipline: Molecular Biology and Biotechnology

Education:

Ph.D.: Department of Biochemistry, Bangalore University

,

Post Doc: Indian Institute of Science, University of Agricultural Sciences, GKVK, Bangalore: 2004-2012

Research Interest

Involved in the management of pod borer, Helicoverpa armigera in Pigeonpea. For this, both transgenic and non-transgenic approaches are being carried out. The transgenic approach for the mitigation of this insect pest is by using different insecticidal proteins (ICPs) from Bacillus thuringiensis using the tissue culture-independent in planta transformation strategy.The core of this programme has been the meristem-based in planta transformation strategy developed by me. The pigeonpea transformants have been identified by various molecular analysis as well as their bioefficacy against the pod borer under both laboratory and nethouse conditions. Selected promising transgenic events will be assessed under field conditions.

The non-transgenic approach to tackle pod borer has been through the expoitation of pigeonpea wild relatives that are resistant to the herbivore. Deciphering of molecular mechanisms to understand the underlying resistance response is underway. For this various omics approaches are being used for the identification of insect resistance genes that could be deployed for the management of pod borer.

Awards & Honors

  • DST – Young Scientist Awardee for Fast Track Project 2004 of Department of Science & Technology titled 'Genetic Transformation in Sunflower and Safflower for resistance against fungal diseases'
  • Best Research Paper Award for the year 2010 in Journal of Plant Science Research
  • Recepient of 2014 Society for Applied Biotechnology award for "Excellencein Plant Genetic Engineering"
  • Member, The National Academy of Sciences, India
  1. Kesiraju, K., Mishra, P., Bajpai, A., Manju Sharma, Uma Rao & Rohini Sreevathsa (2020). Agrobacterium tumefaciens-mediated in planta transformation strategy for development of transgenics in cotton (Gossypium hirsutum L.) with GFP as a visual marker. Physiol Mol Biol Plants 26, 2319–2327. https://doi.org/10.1007/s12298-020-00887-y.
  2. Dutta, Tushar K., Pradeep K. Papolu, Divya Singh, Rohini Sreevathsa, and Uma Rao. (2020). Expression interference of a number of Heterodera avenae conserved genes perturbs nematode parasitic success in Triticum aestivum. Plant Science: 110670.
  3. Tyagi, S., Kesiraju, K., Saakre, M., Rathinam, M., Raman, V., Pattanayak, D., & Sreevathsa, R. (2020). Genome Editing for Resistance to Insect Pests: An Emerging Tool for Crop Improvement. ACS Omega. doi.org/10.1021/acsomega.0c01435ACS
  4. Karthik, K., Nandiganti, M., Thangaraj, A., Singh, S., Mishra, P., Rathinam, M., Sharma, M., Singh, N. K., Dash, P. K., & Sreevathsa, R. (2020). Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance. Frontiers in Plant Science, 11, 768. https://doi.org/10.3389/fpls.2020.00768
  5. Rathinam, M., Roschitzki, B., Grossmann, J., Mishra, P., Kunz, L., Wolski, W., ... & Sreevathsa, R. (2020). Unraveling the proteomic changes involved in the resistance response of Cajanus platycarpus to herbivory by Helicoverpa armigera. Applied Microbiology and Biotechnology, 104, 7603–7618
  6. Kumar, Nikhil Ram, Maniraj Rathinam, Shweta Singh, Karthik Kesiraju, Vikraman Muniyandi, Nagendra Kumar Singh, Prasanta K. Dash, and Rohini Sreevathsa. (2020). Assessment of Pigeonpea (Cajanus cajan L.) transgenics expressing Bt ICPs, Cry2Aa and Cry1AcF under nethouse containment implicated an effective control against herbivory by Helicoverpa armigera (Hübner). Pest Management Science. 76 (5), 1902-1911
  7. Maniraj Rathinam, Uma Rao, Rohini Sreevathsa. (2020). Novel biotechnological strategies to combat biotic stresses: polygalacturonase inhibitor (PGIP) proteins as a promising comprehensive option. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-020-10396-3
  8. Muralimohan, N., Ravi Prakash Saini, Karthik Kesiraju, Debasis Pattanayak, P. Ananda Kumar, K. Kasturi, and Rohini Sreevathsa. (2020) "Molecular stacking of two codon-modified genes encoding Bt insecticidal proteins, Cry1AcF and Cry2Aa for management of resistance development in Helicoverpa armigera." Journal of Plant Biochemistry and Biotechnology. https://doi.org/10.1007/s13562-020-00569-6
  9. Singh, S., Mahato, A.K., Jayaswal, P.K., Singh, N., Dheer, M., Goel, P., Raje, R.S., Yasin, J.K., Sreevathsa, R., Rai, V. and Gaikwad, K., 2020. A 62K genic-SNP chip array for genetic studies and breeding applications in pigeonpea (Cajanus cajan L. Millsp.). Scientific reports, 10(1), pp.1-14.
  10. Khushboo Kumari, Monika Prakash Rai, Navita Bansal, G. Rama Prashat, Sweta Kumari, Rohini Srivathsa, Anil Dahuja, Archana Sachdev, Shelly Praveen and T. Vinutha. (2020) Study of subcellular localization of Glycine max γ-tocopherol methyl transferase isoforms in N. benthamiana. 3 Biotech. 10, 110 https://doi.org/10.1007/s13205-020-2086-9.
  11. Bhupal Hatzade, Rohini Sreevathsa1, Udayakumar Makarla2 and Uma Rao. (2019). Evaluation of activation tagged rice mutants for variability in response to Meloidogyne graminicola under challenged inoculation. Indian J. Genet., 79(4) 685-692.
  12. Bernd Roschitzki, Jonas Grossmann, Paolo Nanni, Christian Panse, Witold Wolski, Maniraj Rathinam, Pragya Mishra, Vandna Rai, Ralph Schlapbach, Nagendra Kumar Singh, Uma Rao, Rohini Sreevathsa. (2019). An integrative outlook at protein and RNA level to understand the mechanism of resistance in a pigeonpea wild relative, Cajanus platycarpus to herbivory by Helicoverpa armigera (Abstr). Molecular Plant-Microbe Interactions 32:S1.1. https://doi.org/10.1094/MPMI-32-10-S1.1
  13. Kumari, K., Rai, M.P., Bansal, N., Prashat, G.R., Kumari, S., Krishnan, V., Sreevathsa, R., Dahuja, A., Sachdev, A., Praveen, S. and Vinutha, T., 2019. Analysis of γ-Tocopherol methyl transferase3 promoter activity and study of methylation patterns of the promoter and its gene body. Plant Physiology and Biochemistry, 144, pp.375-385.
  14. Chatterjee, Madhurima, Jyoti Yadav, Sengottaiyan Vennila, P. R. Shashank, Nisha Jaiswal, Rohini Sreevathsa, and Uma Rao. "Diversity analysis reveals genetic homogeneity among Indian populations of legume pod borer, Maruca vitrata (F.)." 3 Biotech 9, no. 9 (2019): 319.
  15. Hatzade, Bhupal, Rohini Sreevathsa, Udayakumar Makarla, and Uma Rao. "T-DNA activation tagging in rice results in a variable response to Meloidogyne graminicola infection." Biologia (2019): 1-21.
  16. Rathinam, Maniraj, Pragya Mishra, Ajay Kumar Mahato, Nagendra Kumar Singh, Uma Rao, and Rohini Sreevathsa. "Comparative transcriptome analyses provide novel insights into the differential response of Pigeonpea (Cajanus cajan L.) and its wild relative (Cajanus platycarpus (Benth.) Maesen) to herbivory by Helicoverpa armigera (Hübner)." Plant Molecular biology (2019): 1-20. Volume 101, Issue 1–2, pp 163–182
  17. Rathinam, Maniraj, Pragya Mishra, Madavan Vasudevan, Roli Budhwar, Ajay Mahato, A. Lakshmi Prabha, Nagendra Kumar Singh, Uma Rao, and Rohini Sreevathsa. Comparative transcriptome analysis of pigeonpea, Cajanus cajan (L.) and one of its wild relatives Cajanus platycarpus (Benth.) Maesen. PloS one 14, no. 7 (2019): e0218731.
  18. Reddy, Sowmya H., Sumanth K. Kambalimath, Rajesh K. Singhal, Manjunath K. Chikkakariyappa, Raveendran Muthurajan, Mavinahalli P. Rajanna, Rohini Sreevathsa et al. Allele‐specific analysis of single parent backcross population identifies HOX10 transcription factor as a candidate gene regulating rice root growth.  Physiologia Plantarum 166: 596–611. 2019.
  19. Sufia Farhat, Neha Jain, Nisha Singh, Rohini Sreevathsa, Prasanta K. Dash, Rhitu Rai, Sandeep Yadav, Pramod Kumar, Anand Sarkar, Ajay Jain, Nagendra K Singh, Vandna Rai. CRISPR-Cas9 directed genome engineering for enhancing salt stress tolerance in rice. Seminars in Cell and Developmental Biology, https://doi.org/10.1016/j.semcdb.2019.05.003.
  20. Rathinam, M., Kesiraju, K., Singh, S., Thimmegowda, V., Rai, V., Pattanayak, D., & Sreevathsa, R. (2019). Molecular Interaction-Based Exploration of the Broad Spectrum Efficacy of a Bacillus thuringiensis Insecticidal Chimeric Protein, Cry1AcF. Toxins, 11(3), 143.
  21. Mishra P, Jain A, Takabe T, Tanaka Y, Negi M, Singh N, Jain N, Mishra V, Maniraj R, Krishnamurthy SL, Sreevathsa R, Singh NK, Rai V. Heterologous Expression of Serine Hydroxymethyltransferase-3 From Rice Confers Tolerance to Salinity Stress in E. coli and Arabidopsis. Front Plant Sci. 2019 Mar 19; 10:217. doi: 10.3389/fpls.2019.00217. PubMed PMID: 30941150; PubMed Central PMCID: PMC6433796.
  22. Singh, S., Kumar, N. R., Maniraj, R., Lakshmikanth, R., Rao, K. Y. S., Muralimohan, N., ...  Pattanayak, D and Rohini Sreevathsa (2018). Expression of Cry2Aa, a Bacillus thuringiensis insecticidal protein in transgenic pigeon pea confers resistance to gram pod borer, Helicoverpa armigera. Scientific reports, 8(1), 8820.
  23. Saini, R. P., Raman, V., Dhandapani, G., Malhotra, E. V., Sreevathsa, R., Kumar, P. A., ... & Pattanayak, D. (2018). Silencing of HaAce1 gene by host-delivered artificial microRNA disrupts growth and development of Helicoverpa armigera. PloS one, 13(3), e0194150.
  24. Sreeman, S., Vijayaraghavareddy, P., Sreevathsa, R., Rajareddy, S., Arakesh, S., Bharti, P., & Soolanayakanahally, R. (2018). Introgression of physiological traits for a comprehensive improvement of drought adaptation in crop plants. Frontiers in Chemistry, 6, 92.
  25. Bansal, N., Kumari, K., Sreevathsa, R., Krishnan, V., Kumari, S., Dahuja, A., ... & Praveen, S. (2017). Comparative analysis of tocopherol biosynthesis genes and its transcriptional regulation in soybean seeds. Journal of Agricultural and Food Chemistry 65 (50), 11054-11064.
  26. Karthik K, Rohini S. Apical Meristem-Targeted In Planta Transformation Strategy: an Overview on its Utility in Crop Improvement. (2017) Agri Res & Tech: Open Access J. 2017; 8(2): 555734. DOI: 10.19080/ARTOAJ.2017.08.555734.
  27. Ganguly, S., Ghosh, G., Purohit, A., Sreevathsa, R., Chaudhuri, R. K., & Chakraborti, D. (2017). Effective Screening of Transgenic Pigeonpea in Presence of Negative Selection Agents. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 1-7.
  28. Maniraj Rathinam, Shweta Singh, Debasis Pattanayak and Rohini Sreevathsa (2017). Comprehensive in silico allergenicity assessment of novel protein engineered chimeric cry proteins for safe deployment in crops. BMC Biotechnol. 2017; 17: 64
  29. Raman KV, Aggarwal D, Rao SR, Sreevathsa R, Singh AK,  Abdin MZ, Pattanayak D and Mohapatra T. (2018). Rapid and efficient Agrobacterium mediated transformation of early scutellum derived calli of indica rice. Indian Journal of Experimental Biology. 56: 20-28.
  30. Ravi Prakash Saini, Era Vaidya Malhotra, Rohini Sreevathsa, P. Ananda Kumar, Debasis Pattanayak and T. R. Sharma (2017). Two acetylcholinesterase isoforms genes, HaAce1 and HaAce2, express differentially in various developmental stages of Helicoverpa armigera. Indian Res. J. Genet. & Biotech. 9(2): 317 – 323.
  31. Tagginahalli N. Shivakumara, Rohini Sreevathsa, Prasanta K Dash, M.S Sheshshayee, Pradeep K. Papolu, Uma Rao, Narendra Tuteja and M. Udaya Kumar. (2017) Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.). Sci. Rep. 7: 2760 | DOI:10.1038/s41598-017-02589-0.
  32. Tagginahalli N. Shivakumara, Sonam Chaudhary, Divya Kamaraju, Tushar K. Dutta, Pradeep K. Papolu, Prakash Banakar, Rohini Sreevathsa, Bhupinder Singh, K. M. Manjaiah and Uma Rao. (2017). Host-Induced Silencing of Two Pharyngeal Gland Genes Conferred Transcriptional Alteration of Cell Wall-Modifying Enzymes of Meloidogyne incognita vis-à-vis Perturbed Nematode Infectivity in Eggplant. Front. Plant Sci., 30 March 2017
  33. Pragya Mishra, Shweta Singh, Maniraj Rathinam, Muralimohan Nandiganti, Nikhil Ram Kumar, Arulprakash Thangaraj,  Vinutha Thimmegowda, Veda Krishnan, Vagish Mishra, Neha Jain, Vandna Rai, Debasis Pattanayak and Rohini Sreevathsa (2017) Comparative proteomic and nutritional composition analysis of independent transgenic pigeon pea seeds harboring cry1AcF and cry2Aa genes and their non-transgenic counterparts. Journal of Agriculture and Food Chemistry 65 (7), pp 1395–1400. DOI: 10.1021/acs.jafc.6b05301.
  34. Aftab Hussain IS, Pavithra HV, Rohini Sreevathsa, Nataraja KN and Naveen Babu (2016). Development of transgenic pigeon pea (Cajanus cajan L.) overexpressing citrate synthase gene for high phosphorous uptake. Indian Journal of Experimental Biology 54: 493-501.
  35. Sundaresha S, Rohini Sreevathsa, Appanna VK, Manoj-Kumar Arthikala, Shanmugam NB, Shashibhushan NB, Hari Kishore CM, Pannerselvam R,  Kirti PB and Udayakumar M. (2016). Co-overexpression of Brassica juncea NPR1 (BjNPR1) and Trigonella foenum-graecum defensin (Tfgd) in transgenic peanut provides comprehensive but varied protection against Aspergillus flavus and Cercospora arachidicola. Plant Cell Rep. 35:1189–1203 DOI 10.1007/s00299-016-1945-7.
  36. Vemanna V. S, Swetha TN, Shekarappa H. Sheela, Chndrashekar K. Babitha , Sreevathsa Rohini, Malireddy K.Reddy, Narendra Tuteja, Chandrashekar P. Reddy, Trichi Ganesh Prasad and Makarla Udayakumar. (2016). Simultaneous expression of regulatory genes associated with specific drought adaptive traits improves drought adaptation in peanut. Plant Biotechnology Journal. 14: 1008–1020 DOI 10.1111/pbi.1246.
  37. Sushil Chhapekar, Sanagala Raghavendrarao, Gadamchetty Pavan, Chopperla Ramakrishna, Vivek Kumar Singh, Mullapudi Lakshmi Venkata Phanindra, Gurusamy Dhandapani, Rohini Sreevathsa, Polumetla Ananda Kumar (2014). Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate. Plant Cell Reports DOI 10.1007/s00299-014-1732-2
  38. Vivek Kumar Singh, Sellamuthu Gothandapani, Mullapudi Lakshmi Venkata Phanindra, Vikrant Nain, Rohini Sreevathsa, K R S Sambasiva Rao and Polumetla Ananda Kumar. (2014). Vacuolar Targeting of Cry1Ac and its Effects on Expression and Stability in Tobacco. Current Trends in Biotechnology and Pharmacy. 8 (3) 276-287.
  39. Manoj-Kumar Arthikala, Kalpana Nanjareddy, Miguel Lara, Rohini Sreevathsa (2014). Utility of a tissue culture-independent Agrobacterium-mediated in planta transformation strategy in bell pepper to develop fungal disease resistant plants. Scientia Horticulturae 170 (2014) 61–69.
  40. Israr Ahmad, A.U. Solanke, D.P. Deshmukh, M. Kanakachari, Rohini Sreevathsa, D. Pattanayak, T.R. Sharma and P.A. Kumar (2014). Isolation and characterization of Ethylene Responsive Factor (ERF) genes from brinjal. Indian J. Hort. 71(1), 49-54.
  41. Pradeep Kumar Papolu, Nagavara Prasad Gantasala, Divya Kamaraju, Prakash Banakar, Rohini Sreevathsa, Uma Rao (2013) Utility of Host Delivered RNAi of Two FMRF Amide Like Peptides, flp-14 and flp-18, for the Management of Root Knot Nematode, Meloidogyne incognita. PLOS ONE 8(11) e80603.
  42. Nagavara Prasad Gantasala, Pradeep Kumar Papolu, Prasoon Kumar Thakur, Divya Kamaraju, Rohini Sreevathsa  and Uma Rao (2013) Selection and validation of reference genes forquantitative gene expression studies by real-time PCR in eggplant (Solanum melongena L). BMC Research Notes 6: 312
  43. Mahesh Patil,  S. V. Ramu, P. Jathish, Rohini Sreevathsa, P. Chandrashekar Reddy, T. G. Prasad,  M. Udayakumar. (2014). Overexpression of AtNAC2 (ANAC092) in groundnut (Arachis hypogaea L.) improves abiotic stress tolerance. Plant Biotechnol Rep. DOI 10.1007/s11816-013-0305-0; 8: 161-169.
  44. M. Manjulatha, Rohini Sreevathsa, A. Manoj Kumar, Chinta Sudhakar, T. G. Prasad, Narendra Tuteja and M. Udayakumar. Overexpression of a Pea DNA Helicase (PDH45) in Peanut (Arachis hypogaea L.) Confers Improvement of Cellular Level Tolerance and Productivity Under Drought Stress (2014). Molecular Biotechnology. DOI 10.1007/s12033-013-9687-z; 56: 111-125.
  45. Keshavareddy G., Rohini Sreevathsa, S. V. Ramu,  Sundaresha S, A. R. V. Kumar, P. Ananda Kumar & Udayakumar M  (2013). Transgenics in groundnut (Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.). Physiology and molecular biology of plants. DOI 10.1007/s12298-013-0182-6.
  46. Manjunath KC, Mahadeva A., Rohini Sreevathsa, Ramachandra N., Prasad TG (2013). In Vitro Screening and Identification of Putative Sunflower (Helianthus annus L.) Transformants Expressing ECNAC1 Gene by Salt Stress Method. Trends in Biosciences. 6 (1): 108-111.
  47. Prajna Hegde, Vitobha Malaiyappa Choudki, Naveen Babu, Sashidhar Vudayagiri Radhakrishnan and Rohini Sreevathsa (2012). Strategies to Co-express Two Genes Encoding Functionally Related Proteins for Osmolyte Biosynthesis and Ion Homeostasis: Does the Conventional Crossing Method have an Advantage over the Tissue Culture Technique to Develop Co-expressed Transgenics for Stress Tolerance? LS-An international Journal for Plant Sciences. 1(3): 192-200.
  48. Sundaresha S., Rohini Sreevathsa, Gurupada B. Balol, G. Keshavareddy, K. T. Rangaswamy & M. Udayakumar. (2012). A simple, novel and high efficiency sap inoculation method to screen for tobacco streak virus. Physiol. Mol. Biol. Plants 18(4): 365-369.
  49. Kalyananagar Chikkakariyappa Manjunath, Rohini Sreevathsa, Narayanaswamy Ramachandra Swamy, Makarala Udaya Kumar. (2011) Expression and specificity of EcNAC1 transcription factor from Eleusine coracana in Escherichia coli. Biotechnol. Bioinf. Bioeng. 1(4):459-464.
  50. Jyothsna P. Rao, Pushpa Agrawal, Riaz Mahmood, Rohini Sreevathsa, K. Sankara Rao, G. R. Reddy and V. V. S. Suryanarayana. (2012). Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics. Physiology and Molecular Biology of Plants. Volume 18, Number 1, 51-57, DOI: 10.1007/s12298-011-0095-1
  51. Sushma M. Awaji, Madhavi D. Naidu, Prashantkumar S. Hanjagi and Sashidhar V. Ramakrishna, Rohini Sreevathsa (2011). Possibility of Improved Salt Tolerance in Rice Transgenics Overexpressing pgNHX1. Genes Genomes and Genomics.
  52. S. V. Ramu, S. Rohini, G. Keshavareddy, M. Gowri Nee- lima, N. B. Shanmugam, A. R. V. Kumar, S. K. Sarangi, P. Ananda Kumar and M. Udayakumar, “Expression of a synthetic cry1AcF Gene in Transgenic Pigeon pea Con-fers Resistance to Helicoverpa armigera,” Journal of Ap-plied Entomology, Vol. 136, No. 9, 2012, pp. 675-687. doi:10.1111/j.1439-0418.2011.01703.x
  53. Keshamma E., Rohini Sreevathsa, Manoj Kumar A., Kalpana N. Reddy, Manjulatha M., Shanmugam N. B.,  Kumar A. R. V. and Udayakumar M. (2012). Agrobacterium-Mediated in Planta Transformation of Field Bean (Lablab purpureus L.) and Recovery of Stable Transgenic Plants Expressing the cry1AcF Gene. Plant Mol. Biol. Rep. (DOI 10.1007/s11105-011-0312-7)  30:67–78
  54. Nagaveni Venkataramaiah, Sashidhar Vudayagiri Ramakrishna and Rohini Sreevathsa. (2011). Overexpression of phytochelatin synthase (AtPCS) in rice for tolerance to cadmium stress. Biologia, Section Cellular and Molecular Biology, 66(6): 1060—1073.
  55. Sundaresha Siddappa, Rohini Sreevathsa, Rangaswamy Kattarigatta Topegowda and Udayakumar Makarla (2011). Strategies for Viral Disease Resistance in Crop Plants. The Asian and Australasian Journal of Plant Science and Biotechnology, pp.73-78.
  56. Manoj Kumar, Kalpana N Reddy, Manjulatha M, Elizabeth S Arellano, Rohini Sreevathsa and Girija Ganeshan (2011). A rapid, novel and high-throughput identification of putative bell pepper transformants generated through in planta approach. Scientia Horticulturae, 129: 898-903.
  57. Prashantkumar Shrishail Hanjagi, Sashidhar Vudayagiri Radhakrishnan, Sushma Muragendra Awaji and Rohini Sreevathsa (2011). Improvement of Salt Tolerance in Putatively Transgenic Rice Plants Overexpressing AVP1, a Vacuolar H+-Pyrophosphatase. Transgenic Plant Journal, pp. 44-49.
  58. Arthikala Manoj Kumar, Rohini Sreevathsa, Kalpana Nanja Reddy, Keshavareddy  Gopalareddy, Prasad Trichy Ganesh and Makarla Udayakumar (2011). Amenability of Castor to an Agrobacterium- Mediated in planta Transformation Strategy Using a cry1AcF Gene for Insect Tolerance. J. Crop Sci. Biotech. 14(2): 1-10.
  59. Manjulatha Mekapogu., Bala Murali Krishna Vasamsetti, Rohini Sreevathsa, Appanna Venkappa Koppad, Suma Tumkur Chandrashekar, Sudhakar Chinta and Devendra R. (2010). Characterization of Groundnut (Arachis hypogaea. L) Transgenics for the Stability and Effectiveness of the Transgene, EPSPS. J. Plant Biol. 37(3):1-6
  60. D. Gayathri, M. P. Chaithanya, Rohini Sreevathsa, M. S. Sheshshayee and T. G. Prasad. (2010). Protocol for the Production of Doubled Haploids from Rice F1 Anthers. J. Plant Biol., 37 (3), pp. 1–7.
  61. Manoj Kumar A., Sundaresha S. and Rohini Sreevathsa (2011). Transgenic Sunflower (Helianthus annuus L.) with Enhanced Resistance to a Fungal Pathogen Alternaria helianthi. Transgenic Plant Journal 5(1): 50-56.
  62. Sundaresha S., Manoj Kumar A., Rohini S., Math S. A., Keshamma E., Chandrashekar S. C. and Udayakumar M. (2010). Enhanced protection against two major fungal pathogens of groundnut, Cercospora arachidicola and Aspergillus flavus in transgenic groundnut over-expressing a tobacco β 1–3 glucanase. European Journal of Plant Pathology, 126: 497–508.
  63. Sushma, M. Awaji, Nagaveni V., Prashantkumar, S. Hanjagi, Madhavi D.N., Sashidhar V.R. and Rohini Sreevathsa (2010). Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as paradigm. Physiol. Mol. Biol. Plants, 16(2): 135-139.
  64. Manoj Kumar A., Sundareshs S. and Rohini Sreevathsa (2009). Resistance to Alternaria leaf spot disease in safflower (Carthamus tinctorius L.) harboring a rice chitinase gene. Transgenic Plant Journal. 3 (special issue): 113-118.
  65. Keshavareddy G., Rohini Sreevathsa, S. Sundaresha, Kumar A. R. V. and Udayakumar M. (2009). Genetic engineering of crop plants: methods for characterization with special reference to Bt transgenic plants. Current Biotica, 3(2): 278-330.
  66. Sowmya Sudhan, Rohini Sreevathsa, Nagaveni V., Ravikumar K. and Sashidhar V. R. (2009). Genetic engineering of rice for heavy metal stress tolerance: Initial physiological screening of diverse rice genotypes and stringent evaluation test (SET) of putative transformants. Journal of Plant Science Research, 25 (1): 21-37.
  67. Sundaresha S., Rohini Sreevathsa, Keshavareddy G. and Udayakumar M. (2009). Agrobacterium tumefaciens - genetics to gene transfer. Current Biotica, 4 : 507-535.
  68. Manoj Kumar A., Kalpana N. Reddy, Rohini Sreevathsa, Girija Ganeshan and Udayakumar M. (2009). Towards crop improvement in bell pepper (Capsicum annuum L.): Transgenics (uid A::hpt II) by a tissue-culture-independent Agrobacterium-mediated in planta approach. Scientia Horticulturae, 119: 362–370.
  69. Manjunatha S. B., Suma T. C., Rohini Sreevathsa, Devendra R. and Udayakumar M. (2009). Development of transgenics in groundnut, Arachis hypogaea L. harboring a modified EPSPS gene. J. Oilseeds Res. 26(special issue) 646-649.
  70. Gowri Neelima M., Rohini Sreevathsa, Kumar A. R. V. and Gayatri M. C. (2009). Agrobacterium-mediated in planta transformation of chickpea: utility of EPSPS as a selectable marker. J. Plant Biol., 36(1&2): 55-63.
  71. Manjunatha B., Suma T.C., Rohini Sreevathsa, Devendra R., Udayakumar M. and Prasad T.G. (2008). Evaluation of advanced generation transgenic groundnut lines resistant to herbicide-glyphosate. Indian Journal of Weed Science, 40 (3&4) : 162-165.
  72. Gowri Neelima M., Ramu S. V., Rohini Sreevathsa, Asha Rani B. M., Kumar A. R. V. and Gayatri M. C. (2008). In planta transformation strategy to generate transgenic plants in chickpea: Proof of concept with a cry gene. J. Plant Biol. 35(3), 201-206.
  73. Keshamma E., Rohini Sreevathsa, Madhusudhan B. and Prasad T. G. (2008). Transformability in field bean (uidA::nptII) by Agrobacterium tumefaciens-mediated in planta strategy. Journal of Plant Biology, 35(1): 31-37.
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Name Designation Education Mobile Number Profile Picture
R Hari Chandana MSc student BSc (Agricultural Sciences)
Samrita Garnaik PhD student MSc (Molecular Biology & Biotechnology)
Kanishk Diwekar PhD student MSc (Molecular Biology & Biotechnology)
Anuj Kumar PhD student MSc (Molecular Biology & Biotechnology)
Muhammed Shamnas v PhD student MSc (Molecular Biology & Biotechnology)
Kanchan BM Singh SRF PhD
Name Designation
Priyanka Kumari MSc
Muhammed Shamnas v MSc
Akash Paul MSc
Amresh Kumar JRF/SRF